30 October – 3 November 2017
- Gert Vriend (CMBI, course coördinator)
- Daniel Hoffmann (University Duisburg/Essen (TBC))
- Hanka Venselaar (CMBI)
- Wouter Touw (CMBI)
Center for Molecular and Biomolecular Informatics (CMBI), Radboud University Nijmegen-Medical Centre /NCMLS, Nijmegen, the Netherlands
The course is suitable for both PhD students working in structural bioinformatics as well as for PhD students in the life sciences who want to learn more about using structural informatioin in their research. The course schedule accommodates both groups, parallel sessions for both groups will be scheduled when necessary.
In the right hands, protein structures are a ‘power’ful tool to answer bio-molecular questions. Knowledge of the structure is a pre-requisite for rational drug design, for biotechnology, for chemical biology, and for answering a whole series of biomedical questions. In this course we will discuss the ‘production’ of protein structures by NMR, X-ray, and homology modelling. These methods all have their pro’s and cons so a certain ‘prowess’ is needed to follow all ‘promises’ and start attacking bio-medical ‘problems’ using protein structures.
The course will be split in three parts:
Part 1) Looking and seeing things in protein structures, learning to operate the software, understanding some of the algorithms.
Part 2) Protein structure determination (prediction) with NMR, X-ray, and homology modelling, and the possibilities and problems that come with each of these three techniques.
Part 3) Applying all that was learned in real-life example studies.
Examples of questions you will be able to answer after follwing the course:
- Why is a person with this mutation sick? Or in other words, how does the molecular phenotype cause a disease state?
- This enzyme converts mannose. Can I make is specificity broader?
- This enzyme doesn’t function in my in vitro assay. Should I add some ions?
- I want to add a tag to my enzyme, should I put it on the N-terminus, the C-terminus, or is something different needed?
- This receptor binds a ligand, but if I look at the structure, that ligand doesn’t fit at all. Can I predict motions that take place upon ligand binding?
Preliminary course schedule
Day 1: Homology modelling seminar (H Venselaar).
- Watching and seeing protein structures
- Homology modelling
- Answering biological/biomedical questions using protein structures and models”.
Day 2: Protein structure comparison seminars (H Venselaar, G Vriend).
- Sequences analysis when structure data is available
- Answering medical questions with sequence-structure alignments
Day 3: Electrostatics and molecular dynamics seminars (D Hoffmann).
- Role of electrostatics
- Answering biological questions with MD
Day 4: Protein structure determination and validation seminars (W. Touw, G Vriend).
- X-ray and NMR structure comparison
- Protein structure validation and implications
Day 5: Own project executed with help from CMBI staff.
For more information about the course you can contact Celia van Gelder.